|
KMID : 0380020060210050360
|
|
Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 5 p.360 ~ p.365
|
|
|
Surface Modification of Nano Porous Silica Particle for Enzyme Immobilization
|
|
Cho Hyung-Min
Kim Jong-Kil
Kim Ho-Kun
Lee Eun-Kyu
|
|
|
Abstract
|
|
|
|
The objectives of this study were to develop nano-pore silica particles and to modify its surface for use as an enzyme immobilization matrix. Sol-gel reaction was used to produce silica particles of various nano pore sizes with hydroxyl groups on their surfaces. The surface was modified with aldehyde that was confirmed by fluorescence imaging. Trypsin was covalently immobilized by reductive amination. Surface density of the immobilized trypsin was ca. 350 μg/m2, which was approximately 17- and 35-fold higher than those from the surfaces with hydroxyl and amine group, respectively. About 90% of the initial enzyme activity was maintained after the 12th use of repeated use. When compared with the commercial matrices, the nano-pore silica particle was superior in terms of immobilization yield and specific activity. This study suggests the nano porous silica particles can be used as enzyme immobilization matrix for industrial applications.
|
|
|
KEYWORD
|
|
|
Immobilization, enzyme activity, surface modification, fluorescence image, nano-pore silica
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|